1Z0P
Crystal structure of the Protein of Unknown Function SPY1572 from Streptococcus pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 67.583, 67.583, 28.592 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.690 - 1.700 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.067 | 0.596 |
| Number of reflections | 16058 | |
| <I/σ(I)> | 41.88 | 2.83 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 10.1 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 20% butanediol, 0.1M acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
