1Z0M
the glycogen-binding domain of the AMP-activated protein kinase beta1 subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-07-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 43.735, 44.891, 50.335 |
Unit cell angles | 70.72, 68.77, 65.79 |
Refinement procedure
Resolution | 30.000 - 1.910 |
R-factor | 0.227 |
Rwork | 0.191 |
R-free | 0.24268 |
Structure solution method | SIR+NCS averaging |
RMSD bond length | 0.015 |
RMSD bond angle | 1.739 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.039 | 0.181 |
Number of reflections | 23738 | |
Completeness [%] | 95.8 | 81.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 299 | PEG, monomethyl ether 5000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 299K |