1Z0A
GDP-Bound Rab2A GTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 98.773, 128.473, 68.236 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.120 |
| R-factor | 0.22171 |
| Rwork | 0.219 |
| R-free | 0.27885 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Polyalanine Rab4 GTPase |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.166 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Number of reflections | 49343 | |
| <I/σ(I)> | 32.9 | 3.7 |
| Completeness [%] | 68.5 | 45.8 |
| Redundancy | 4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 15% PEG 4000, 400mM Lithium sulfate, 100mM Citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
