1Z06
GppNHp-Bound Rab33 GTPase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-02-25 |
Detector | MAR scanner 300 mm plate |
Spacegroup name | P 32 |
Unit cell lengths | 56.194, 56.194, 55.039 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 1.810 |
R-factor | 0.19082 |
Rwork | 0.189 |
R-free | 0.21794 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Polyalanine Rab5c GTPase |
RMSD bond length | 0.008 |
RMSD bond angle | 1.275 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 17431 | |
<I/σ(I)> | 29 | 7.7 |
Completeness [%] | 96.7 | 84.2 |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% PEG 6000, 10% Glycerol, 1M Lithium Chlroride, 50mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |