1YZT
GppNHp-Bound Rab21 GTPase at 2.05 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-04-14 |
Detector | MAR scanner 300 mm plate |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 130.357, 36.567, 86.666 |
Unit cell angles | 90.00, 113.40, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.050 |
R-factor | 0.21425 |
Rwork | 0.210 |
R-free | 0.28586 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Polyalanine Rab3a GTPase |
RMSD bond length | 0.010 |
RMSD bond angle | 1.260 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.110 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 23946 | |
<I/σ(I)> | 15.8 | 3.8 |
Completeness [%] | 99.8 | 98.1 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 18% PEG 6000, 200mM Lithium sufate, 50mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |