1YZI
A novel quaternary structure of human carbonmonoxy hemoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-26 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 61.464, 61.464, 174.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.000 - 2.070 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hho |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.200 |
| Data reduction software | bioteX |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 2.200 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.067 | 0.253 |
| Number of reflections | 21330 | |
| <I/σ(I)> | 13.1 | 2 |
| Completeness [%] | 95.2 | 76.7 |
| Redundancy | 5.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 7.1 | 298 | sodium/potassium phosphate, toluene, pH 7.1, SMALL TUBES, temperature 298K |
