1YZ7

Crystal structure of a C-terminal segment of the alpha subunit of aIF2 from Pyrococcus abyssi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	P 21 21 2
Unit cell lengths	61.538, 79.840, 47.941
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.260
R-factor	0.239
Rwork	0.236
R-free	0.28500
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.320
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.000	2.320
High resolution limit [Å]	2.260	2.260
Rmerge	0.047	0.307
Number of reflections	11549
<I/σ(I)>	10.4	2.4
Completeness [%]	99.7	100
Redundancy	3.4	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	297	ammonium sulfate, isopropanol, NaCl, MOPS, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 297K
1	VAPOR DIFFUSION, SITTING DROP	6.7	297	ammonium sulfate, isopropanol, NaCl, MOPS, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 297K