1YZ0

R-State AMP Complex Reveals Initial Steps of the Quaternary Transition of Fructose-1,6-bisphosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2001-02-15
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	59.740, 166.140, 78.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.070
R-factor	0.2181
Rwork	0.218
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eyk
RMSD bond length	0.006
RMSD bond angle	1.200
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.380	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.053	0.272
Number of reflections	53756
<I/σ(I)>	6.7	1.9
Completeness [%]		96.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	A54L FBPase (10mg/ml), 25mM Hepes pH7.4, 5 mM MgCl2, 5mM F16P2, 5mM AMP and a precipitant solution [Hepes (100mM, pH 7.4), PEG 3350 (14% w/v), 2-methyl-2,4-pentanediol (21% v/v), and t-butanol (5% v/v)], VAPOR DIFFUSION, HANGING DROP, temperature 298K