1YYZ
R-State AMP Complex Reveals Initial Steps of the Quaternary Transition of Fructose-1,6-bisphosphatase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2001-02-15 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 53.780, 82.560, 166.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.850 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cnq |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.080 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.035 | 0.211 |
Number of reflections | 32998 | |
<I/σ(I)> | 10.7 | 2.3 |
Completeness [%] | 99.1 | 91.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | A54L FBPase (10 mg/ml), Hepes (25 mM, pH 7.4), MgCl2 (5 mM), F16P2 (5mM), AMP(5mM) and a precipitant solution [Hepes (100 mM, pH7.4), PEG 3350 (12% w/v), glycerol (23% v/v) and t-butanol (5% v/v)], VAPOR DIFFUSION, HANGING DROP, temperature 293K |