1YYK
Crystal structure of RNase III from Aquifex Aeolicus complexed with double-stranded RNA at 2.5-angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-14 |
Detector | BRUKER PROTEUM 300 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.270, 61.950, 131.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.080 - 2.500 |
R-factor | 0.23 |
Rwork | 0.226 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 1I4S |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.080 | 2.660 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.148 | 0.367 |
Number of reflections | 21859 | |
<I/σ(I)> | 9.1 | 1.1 |
Completeness [%] | 88.0 | 57 |
Redundancy | 4.8 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 292 | Magnesium formate, PEG3350, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Magnesium formate | ||
2 | 1 | 1 | PEG3350 | ||
3 | 1 | 1 | H2O | ||
4 | 1 | 2 | Magnesium formate | ||
5 | 1 | 2 | PEG3350 | ||
6 | 1 | 2 | H2O |