1YY5
Crystal structure of Fms1, a polyamine oxidase from Yeast
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9700 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 102.711, 215.365, 118.157 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.249 |
Rwork | 0.249 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.367 | |
Number of reflections | 56554 | |
Completeness [%] | 96.7 | 78.6 |
Redundancy | 6.5 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 296 | 10% PEG3350, 100mM Hepes, 200mM CaCL2, pH7.0, VAPOR DIFFUSION, SITTING DROP, temperature 296K |