1YY3

Structure of S-Adenosylmethionine:tRNA Ribosyltransferase-Isomerase (QueA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9393
Spacegroup name	P 4 21 2
Unit cell lengths	101.671, 101.671, 152.794
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.000 - 2.880
R-factor	0.364
Rwork	0.364
R-free	0.38600
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	41787
<I/σ(I)>	27.9	2.6
Completeness [%]	99.9	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG4000, ammonium sulphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG4000, ammonium sulphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG4000, ammonium sulphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K