1YXY
Crystal Structure of putative N-acetylmannosamine-6-P epimerase from Streptococcus pyogenes (APC29713) Structural genomics, MCSG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-07 |
Detector | SBC-3 |
Wavelength(s) | 0.979484 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.640, 83.521, 104.256 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.600 |
R-factor | 0.19407 |
Rwork | 0.193 |
R-free | 0.20717 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.026 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.123 | 0.809 |
Number of reflections | 68381 | |
<I/σ(I)> | 26 | 2.4 |
Completeness [%] | 98.4 | 98.2 |
Redundancy | 13 | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 273 | ammonium sulfate, BIS-TRIS buffer, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 273K |