1YXQ
Crystal structure of actin in complex with swinholide A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-29 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.000, 76.800, 98.400 |
| Unit cell angles | 90.00, 101.20, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.010 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.648 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP (7.0.0) |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.055 | 0.301 |
| Number of reflections | 66151 | |
| <I/σ(I)> | 24.9 | 3.6 |
| Completeness [%] | 99.4 | 95.7 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | small-scale batch | 8.5 | 277 | dimethyl PEG 5000, HEPPS, MgCl2, TCEP, NaN3, pH 8.5, small-scale batch, temperature 277K |
