1YX2
Crystal Structure of the Probable Aminomethyltransferase from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-17 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.628, 88.095, 88.764 |
| Unit cell angles | 90.00, 101.78, 90.00 |
Refinement procedure
| Resolution | 41.200 - 2.080 |
| R-factor | 0.21916 |
| Rwork | 0.212 |
| R-free | 0.28797 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.447 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.200 | 2.180 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.114 | 0.432 |
| Number of reflections | 41510 | |
| <I/σ(I)> | 6.8 | 2.4 |
| Completeness [%] | 81.7 | |
| Redundancy | 6.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | ammonium sulfate, HEPES, PEG, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
