1YWB
0.9 A Structure of NP4 from Rhodnius Prolixus complexed with NO at pH 5.6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-30 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.900 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 69.989, 42.529, 52.959 |
Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
Resolution | 28.000 - 0.970 |
R-factor | 0.141 |
Rwork | 0.140 |
R-free | 0.17000 |
Starting model (for MR) | PDB ID 1KOI |
RMSD bond length | 0.014 |
RMSD bond angle | 0.033 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 1.010 |
High resolution limit [Å] | 0.970 | 0.970 |
Number of reflections | 87670 | |
<I/σ(I)> | 7.6 | 2.7 |
Completeness [%] | 96.0 | 93 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | 2.8 M ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |