1YW1
Structure Of Ytnj From Bacillus Subtilis in complex with FMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | .979 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 86.254, 86.254, 194.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.810 |
| R-factor | 0.18553 |
| Rwork | 0.182 |
| R-free | 0.25241 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.926 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.095 | 0.350 |
| Number of reflections | 17468 | |
| <I/σ(I)> | 13.1 | 2.5 |
| Completeness [%] | 93.3 | 94.2 |
| Redundancy | 5.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 1.5M NACITRATE, 0.1M HEPES PH 7.5, soaked in .01M FMN, CRYO: 30% glucose, VAPOR DIFFUSION, temperature 293K |
