1YVY
Crystal structure of Anaerobiospirillum succiniciproducens phosphoenolpyruvate carboxykinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2004-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.659, 55.734, 139.397 |
| Unit cell angles | 90.00, 93.77, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.350 |
| R-factor | 0.219 |
| Rwork | 0.221 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.050 |
| RMSD bond angle | 8.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 35963 | |
| <I/σ(I)> | 8.5 | 1.9 |
| Completeness [%] | 98.8 | 98.8 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 296 | PEG MME 5000, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
