1YVT
The high salt (phosphate) crystal structure of CO Hemoglobin E (Glu26Lys) at physiological pH (pH 7.35)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-12-20 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 53.502, 53.502, 191.958 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.410 - 1.800 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rvw |
RMSD bond length | 0.014 |
RMSD bond angle | 1.600 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.112 | 0.457 |
Number of reflections | 26762 | |
<I/σ(I)> | 17.4 | 4.1 |
Completeness [%] | 99.7 | 100 |
Redundancy | 9.8 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.35 | 289 | 1.65M potassium phosphate, 0.1M HEPES, ph 7.35, Batch Method, temperature 289K |