1YVS
Trimeric domain swapped barnase
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 1996-08 |
Detector | MARRESEARCH |
Spacegroup name | H 3 2 |
Unit cell lengths | 70.080, 70.080, 162.550 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.200 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1brn |
RMSD bond length | 0.011 |
RMSD bond angle | 24.490 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.900 | 2.230 |
High resolution limit [Å] | 2.200 | 2.170 |
Rmerge | 0.069 * | 0.158 * |
<I/σ(I)> | 17 | 4.7 |
Completeness [%] | 98.0 | 95 |
Redundancy | 22 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.5 | pH 4.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | 50 (mM) | ||
3 | 1 | drop | exo-guanosine 2',3'-phosphorothioate | 2 (mM) | |
4 | 1 | reservoir | PEG6000 | 30 (%(w/v)) | |
5 | 1 | reservoir | ammonium sulfate | 0.2 (M) |