1YVP
Ro autoantigen complexed with RNAs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2004-08-24 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.300, 153.650, 80.560 |
Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.2021 |
Rwork | 0.202 |
R-free | 0.23800 |
Structure solution method | MIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.269 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 72908 | |
Completeness [%] | 95.0 | 86.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 278 | MPD, sodium acetate, magnesium acetate, DTT, pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | sodium acetate | ||
3 | 1 | 1 | magnesium acetate | ||
4 | 1 | 1 | DTT | ||
5 | 1 | 2 | MPD | ||
6 | 1 | 2 | sodium acetate | ||
7 | 1 | 2 | magnesium acetate | ||
8 | 1 | 2 | DTT |