1YUY

HEPATITIS C VIRUS NS5B RNA-DEPENDENT RNA POLYMERASE GENOTYPE 2a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Wavelength(s)	1.100028
Spacegroup name	C 2 2 21
Unit cell lengths	64.396, 214.417, 123.192
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.350 - 1.900
R-factor	0.214
Rwork	0.214
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.200
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.900
Number of reflections	65644
<I/σ(I)>	12.7
Completeness [%]	97.2
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	15% PEG 8000, 0.2M ammonium sulphate, 80mM sodium citrate pH 6.0, 7% glycerol, 4% 1,6 hexanediol and 1% benzamidine, VAPOR DIFFUSION, HANGING DROP, temperature 298K