1YUO
Optimisation of the surface electrostatics as a strategy for cold adaptation of uracil-DNA N-glycosylase (UNG)from atlantic cod (Gadus morhua)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM1A |
Synchrotron site | ESRF |
Beamline | BM1A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-03-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.91 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.511, 54.771, 78.071 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.950 |
Rwork | 0.210 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 1AKZ |
RMSD bond length | 0.006 |
RMSD bond angle | 1.233 |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.166 | 0.399 |
Number of reflections | 16602 | |
<I/σ(I)> | 4 | 2.1 |
Redundancy | 2.3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | Sodium cacodylate, Sodium Acetate, PEG 8000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |