1YUM
Crystal Structure of Nicotinic Acid Mononucleotide Adenylyltransferase from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.85 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.179, 110.655, 65.203 |
Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.700 |
Rwork | 0.191 |
R-free | 0.25060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | apo NaMN AT |
RMSD bond length | 0.013 |
RMSD bond angle | 0.028 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.100 | 0.266 |
Number of reflections | 118041 | |
<I/σ(I)> | 23.5 | |
Completeness [%] | 96.8 | 98.9 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | bis-tris propane, trisodium citrate, glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |