1YUK
The crystal structure of the PSI/Hybrid domain/ I-EGF1 segment from the human integrin beta2 at 1.8 resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97600, 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.600, 31.818, 74.950 |
| Unit cell angles | 90.00, 91.20, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.800 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.25300 |
| Structure solution method | SAD |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.062 |
| Number of reflections | 37606 |
| <I/σ(I)> | 20.18 |
| Completeness [%] | 92.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 18% PEG 8000, 0.2M calcium acetate, 0.1M Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
