1YU2
Major Tropism Determinant M1 Variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Wavelength(s) | 1.5479 |
| Spacegroup name | P 63 |
| Unit cell lengths | 93.534, 93.534, 103.146 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.640 - 1.860 |
| R-factor | 0.16384 |
| Rwork | 0.163 |
| R-free | 0.17761 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yu0 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.986 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 42618 | |
| Completeness [%] | 98.8 | 85.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | Isopropanol, Sodium Citrate, HEPES, pH 7.5, VAPOR DIFFUSION, temperature 298K |
