1YTY
Structural basis for recognition of UUUOH 3'-terminii of nascent RNA pol III transcripts by La autoantigen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-10-28 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 153.690, 54.001, 56.684 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.290 |
R-factor | 0.22213 |
Rwork | 0.219 |
R-free | 0.28829 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.726 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.148 | 0.451 |
Number of reflections | 20734 | |
Completeness [%] | 99.8 | 98.5 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | PEG 3350, lithium sulfate, potassium chloride, acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 3350 | ||
10 | 1 | 2 | H2O | ||
2 | 1 | 1 | lithium sulfate | ||
3 | 1 | 1 | potassium chloride | ||
4 | 1 | 1 | acetate | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | PEG 3350 | ||
7 | 1 | 2 | lithium sulfate | ||
8 | 1 | 2 | potassium chloride | ||
9 | 1 | 2 | acetate |