1YTW
YERSINIA PTPASE COMPLEXED WITH TUNGSTATE
Experimental procedure
Detector technology | AREA DETECTOR |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.300, 49.800, 100.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.000 - 2.400 |
R-factor | 0.179 |
Rwork | 0.179 |
Structure solution method | MIR |
RMSD bond length | 0.019 |
RMSD bond angle | 25.200 * |
Data reduction software | MADNES |
Data scaling software | XSCALE |
Phasing software | PHASES |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 9999.000 * | |
High resolution limit [Å] | 2.400 * | 2.400 * |
Rmerge | 0.107 | 0.274 * |
Number of reflections | 9875 | |
Completeness [%] | 89.0 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8.5 | 296 | THE CATALYTIC DOMAIN (RESIDUES 163 - 468) OF YOP51 WAS CRYSTALLIZED AT 20 MG/ML WITH 1 MM SODIUM TUNGSTATE AT 23 DEGREES CELSIUS, BY HANGING DROP VAPOR DIFFUSION AGAINST A WELL SOLUTION OF 22% POLYETHYLENE GLYCOL (MW 4000), 200 MM LITHIUM SULFATE, 10% ISOPROPANOL, 100 MM TRIS HCL (PH 8.5) AND 0.1% BETA-MERCAPTOETHANOL., vapor diffusion - hanging drop, temperature 296K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | PTPase | 20 (mg/ml) | |
2 | 1 | drop | sodium tungstate | 1 (mM) | |
3 | 1 | drop | precipitant | ||
4 | 1 | reservoir | PEG4000 | 22 (%(w/v)) | precipitant |
5 | 1 | reservoir | 200 (mM) | precipitant | |
6 | 1 | reservoir | isopropanol | 10 (%) | precipitant |
7 | 1 | reservoir | beta-mercaptoethanol | 0.1 (%) | precipitant |
8 | 1 | reservoir | Tris-HCl | 100 (mM) | precipitant |