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1YTW

YERSINIA PTPASE COMPLEXED WITH TUNGSTATE

Experimental procedure
Detector technologyAREA DETECTOR
DetectorXUONG-HAMLIN MULTIWIRE
Spacegroup nameP 21 21 21
Unit cell lengths56.300, 49.800, 100.600
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution7.000 - 2.400
R-factor0.179
Rwork0.179
Structure solution methodMIR
RMSD bond length0.019
RMSD bond angle25.200

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Data reduction softwareMADNES
Data scaling softwareXSCALE
Phasing softwarePHASES
Refinement softwareX-PLOR (3.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]9999.000

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High resolution limit [Å]2.400

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2.400

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Rmerge0.1070.274

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Number of reflections9875
Completeness [%]89.0
Redundancy4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

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8.5296THE CATALYTIC DOMAIN (RESIDUES 163 - 468) OF YOP51 WAS CRYSTALLIZED AT 20 MG/ML WITH 1 MM SODIUM TUNGSTATE AT 23 DEGREES CELSIUS, BY HANGING DROP VAPOR DIFFUSION AGAINST A WELL SOLUTION OF 22% POLYETHYLENE GLYCOL (MW 4000), 200 MM LITHIUM SULFATE, 10% ISOPROPANOL, 100 MM TRIS HCL (PH 8.5) AND 0.1% BETA-MERCAPTOETHANOL., vapor diffusion - hanging drop, temperature 296K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropPTPase20 (mg/ml)
21dropsodium tungstate1 (mM)
31dropprecipitant
41reservoirPEG400022 (%(w/v))precipitant
51reservoir200 (mM)precipitant
61reservoirisopropanol10 (%)precipitant
71reservoirbeta-mercaptoethanol0.1 (%)precipitant
81reservoirTris-HCl100 (mM)precipitant

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PDB entries from 2024-03-27

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