1YTN
HYDROLASE
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1994-11-17 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.700, 49.700, 99.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.400 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 1ytw |
| RMSD bond length | 0.020 |
| RMSD bond angle | 22.500 * |
| Data reduction software | MADNES |
| Data scaling software | XSCALE |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9999.000 * | |
| High resolution limit [Å] | 2.400 * | 2.400 * |
| Rmerge | 0.115 | 0.226 * |
| Number of reflections | 9205 | |
| Completeness [%] | 84.0 | 66 * |
| Redundancy | 2.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.2 | 296 | THE CATALYTIC DOMAIN (RESIDUES 163 - 468) OF YOP51 WAS CRYSTALLIZED AT 23 DEGREES CELSIUS BY THE HANGING DROP VAPOR DIFFUSION METHOD. DROP WAS COMPOSED OF EQUAL VOLUMES OF PROTEIN (23 MG/ML) AND RESERVOIR SOLUTION. RESERVOIR SOLUTION WAS 27% POLYETHYLENE GLYCOL (MW 1500), 10% 2-METHYL-2,4-PENTANEDIOL, 220 MM SODIUM NITRATE, 0.1% BETA-MERCAPTOETHANOL, AND 10 MM IMIDAZOLE, PH 7.2., vapor diffusion - hanging drop, temperature 296K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PTPase | 23 (mg/ml) | |
| 2 | 1 | drop | precipitant | ||
| 3 | 1 | reservoir | PEG1500 | 27 (%(w/v)) | precipitant |
| 4 | 1 | reservoir | MPD | 10 (%) | precipitant |
| 5 | 1 | reservoir | beta-mercaptoethanol | 0.1 (%) | precipitant |
| 6 | 1 | reservoir | sodium nitrate | 220 (mM) | precipitant |
| 7 | 1 | reservoir | imidazole | 10 (mM) | precipitant |
