1YTL
Crystal Structure of Acetyl-CoA decarboxylase/synthase complex epsilon subunit 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-22 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.664, 61.944, 71.198 |
| Unit cell angles | 93.92, 103.64, 94.14 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.17306 |
| Rwork | 0.171 |
| R-free | 0.21408 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.808 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.047 | 0.567 |
| Number of reflections | 51787 | |
| Completeness [%] | 92.1 | 72.7 |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | MgCl, Tris Cl, PEG 5K MME, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
