1YTJ

SIV PROTEASE CRYSTALLIZED WITH PEPTIDE PRODUCT

Experimental procedure

Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	62.300, 32.100, 96.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.500
R-factor	0.174
Rwork	0.174
R-free	0.28500
RMSD bond length	0.014
RMSD bond angle	26.700 *
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.500 *
Rmerge	0.079
Number of reflections	3500
Completeness [%]	98.0	100 *
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium chloride	4-6 (%sat)
2	1	reservoir	sodium cacodylate	100 (mM)