1YTH
SIV PROTEASE CRYSTALLIZED WITH PEPTIDE PRODUCT
Experimental procedure
Source type | SYNCHROTRON |
Source details | SSRL |
Synchrotron site | SSRL |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.100, 59.300, 61.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.000 - 2.200 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.28300 |
RMSD bond length | 0.014 |
RMSD bond angle | 27.000 * |
Data reduction software | DENZO |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.200 * | |
Rmerge | 0.080 | |
Number of reflections | 9700 | |
Completeness [%] | 96.0 | 93 * |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 42-50 (%sat) | |
2 | 1 | reservoir | sodium acetate | 200 (mM) | |
3 | 1 | reservoir | dithiothreitol | 1 (mM) |