1YTH
SIV PROTEASE CRYSTALLIZED WITH PEPTIDE PRODUCT
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL |
| Synchrotron site | SSRL |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.100, 59.300, 61.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.200 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.28300 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 27.000 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.200 * | |
| Rmerge | 0.080 | |
| Number of reflections | 9700 | |
| Completeness [%] | 96.0 | 93 * |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 42-50 (%sat) | |
| 2 | 1 | reservoir | sodium acetate | 200 (mM) | |
| 3 | 1 | reservoir | dithiothreitol | 1 (mM) |
