1YTE
Crystal structure of a nicotinate phosphoribosyltransferase from Thermoplasma acidophilum, phosphoribosylpyrophosphate bound structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2004-09-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.28230 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 136.061, 136.061, 120.166 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.960 - 2.750 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 16818 | |
Completeness [%] | 96.2 | 97.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1M Tris, 45% MPD, 0.2M ammonium acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |