1YTD
Crystal structure of a nicotinate phosphoribosyltransferase from Thermoplasma acidophilum, Native Structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2003-08-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.10490 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 136.024, 136.024, 119.733 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.950 - 2.800 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.24600 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 16303 | |
Completeness [%] | 97.7 | 97.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1M Tris, 45% MPD, 0.2M ammonium acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |