1YTC

THERMODYNAMIC CYCLES AS PROBES OF STRUCTURE-FUNCTION RELATIONSHIPS IN UNFOLDED PROTEINS

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1992-03-24
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	36.660, 36.660, 138.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.162
Rwork	0.162
RMSD bond length	0.020
RMSD bond angle	0.036 *
Data reduction software	R-AXIS ((MSC))
Phasing software	X-PLOR (3.1)
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.800	1.800 *
Rmerge	0.060
Total number of observations	59573 *
Number of reflections	9141
Completeness [%]	95.5	93.4 *
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	2	sodium phosphate	0.1 (M)
3	1	2		0.3 (M)
4	1	2	dithiothreitol	40 (mM)
5	1	2	ammonium sulfate	90 (%sat)