1YT8

Crystal Structure of Thiosulfate sulfurtransferase from Pseudomonas aeruginosa

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979, 0.976, 0.94
Spacegroup name	P 21 21 2
Unit cell lengths	88.348, 114.476, 69.438
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.040 - 1.900
R-factor	0.185
Rwork	0.185
R-free	0.20900
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.300
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	56427
Completeness [%]	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	PEG 8000, Ammonium sulfate, Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K