1YT4
Crystal structure of TEM-76 beta-lactamase at 1.4 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.03321 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.601, 59.707, 88.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jwp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.570 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.042 | 0.384 |
| Number of reflections | 43334 | |
| <I/σ(I)> | 15.42 | 2.37 |
| Completeness [%] | 98.2 | 97.8 |
| Redundancy | 8.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 296 | sodium potassium phosphate, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
