1YT3
Crystal Structure of Escherichia coli RNase D, an exoribonuclease involved in structured RNA processing
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-07 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.000, 80.100, 103.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.950 - 1.600 |
| R-factor | 0.1961 |
| Rwork | 0.196 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A 1.9 A structure of RNase D obtained by MIR phasing using 5 Hg sites and one Zn site. |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.950 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.044 | 0.240 |
| Number of reflections | 55994 | |
| <I/σ(I)> | 32.8 | 5.7 |
| Completeness [%] | 97.8 | 86.6 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Ammonium Sulfate, Zinc Sulfate, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
