1YSQ
The crystal structure of transcriptional regulator YaiJ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 65.510, 65.510, 88.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| R-factor | 0.19464 |
| Rwork | 0.194 |
| R-free | 0.20748 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.291 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear ((d*trek)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.795 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 20550 | |
| Completeness [%] | 90.2 | 75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 297 | 8% PEG8K, 0.1M NA Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
