1YSO

YEAST CU, ZN SUPEROXIDE DISMUTASE WITH THE REDUCED BRIDGE BROKEN

Experimental procedure

Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	H 3 2
Unit cell lengths	119.330, 119.330, 75.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.730
R-factor	0.182
Rwork	0.182
RMSD bond length	0.012
RMSD bond angle	26.600 *
Data reduction software	XENGEN
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000
High resolution limit [Å]	1.730	1.730 *
Rmerge	0.063
Total number of observations	102086 *
Number of reflections	20002
Completeness [%]	92.4	40.9 *
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	phosphate	50 (mM)
2	1	reservoir	ammonium sulfate	2.0-2.3 (M)
3	1	reservoir	Tris-HCl	50 (mM)
4	1	reservoir		50 (mM)
5	1	reservoir		0.001 (%(w/v))