1YSD
Yeast Cytosine Deaminase Double Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-05 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.350, 54.770, 68.400 |
| Unit cell angles | 90.00, 105.68, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.163 |
| Rwork | 0.163 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.043 | 0.089 |
| Number of reflections | 22096 | |
| <I/σ(I)> | 22.9 | 6.8 |
| Completeness [%] | 99.3 | 96.3 |
| Redundancy | 3.435 | 3.215 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.50 |
