1YSA
THE GCN4 BASIC REGION LEUCINE ZIPPER BINDS DNA AS A DIMER OF UNINTERRUPTED ALPHA HELICES: CRYSTAL STRUCTURE OF THE PROTEIN-DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 118 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS-NICOLET |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.130, 88.540, 59.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.900 |
| R-factor | 0.23 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 3.300 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.099 |
| Total number of observations | 21237 * |
| Number of reflections | 5289 |
| Completeness [%] | 96.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.75 | 22 * | well solution contains twice the concentrations of salts and PEG400 * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 3 | PEG 400 | ||
| 2 | 1 | 1 | PEG 400 | ||
| 3 | 1 | 1 | NACL | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | MES | ||
| 6 | 1 | 1 | SPERMINE_HCL | ||
| 7 | 1 | 2 | WATER | ||
| 8 | 1 | 2 | PEG 400 | ||
| 9 | 1 | 3 | WATER |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | pGCNK58 monomer | 0.95 (mM) | |
| 2 | 1 | drop | DNA | 0.57 (mM) | |
| 3 | 1 | drop | 0.15 (M) | ||
| 4 | 1 | drop | 0.03 (M) | ||
| 5 | 1 | drop | 0.025 (M) | ||
| 6 | 1 | drop | spermine-HCL | 1 (mM) | |
| 7 | 1 | drop | PEG400 | 12 (%(v/v)) |
