1YS7
Crystal structure of the response regulator protein prrA complexed with Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-23 |
Detector | MARRESEARCH |
Wavelength(s) | 0.81 |
Spacegroup name | P 1 |
Unit cell lengths | 37.350, 46.486, 65.554 |
Unit cell angles | 100.33, 102.01, 92.90 |
Refinement procedure
Resolution | 19.480 - 1.580 |
R-factor | 0.18878 |
Rwork | 0.187 |
R-free | 0.22996 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ys6 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.838 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.630 |
High resolution limit [Å] | 1.580 | 1.600 |
Rmerge | 0.065 | 0.712 |
Number of reflections | 55973 | |
<I/σ(I)> | 22.7 | 2.5 |
Completeness [%] | 97.1 | 85.5 |
Redundancy | 4.1 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | PEG8000, Sodium cacodylate, Glycerol, Magnesium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |