1YS3
Crystal Structure of the ATP binding domain of PrrB from Mycobacterium Tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00 |
Spacegroup name | P 65 |
Unit cell lengths | 61.476, 61.476, 211.333 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.970 - 1.900 |
R-factor | 0.18851 |
Rwork | 0.186 |
R-free | 0.23523 |
Structure solution method | SAD |
RMSD bond length | 0.022 |
RMSD bond angle | 1.901 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 32808 | |
<I/σ(I)> | 31.5 | 2 |
Completeness [%] | 97.7 | 87.1 |
Redundancy | 8.27 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | PEG200DME, Ca acetate, Bis Tris Propane, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | PEG200DME, Ca acetate, Bis Tris Propane, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |