1YRX

Structure of a novel photoreceptor: the BLUF domain of AppA from Rhodobacter sphaeroides

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	105
Detector technology	CCD
Collection date	2004-06-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9, 0.97939, 0.97962, 0.96113
Spacegroup name	P 41 21 2
Unit cell lengths	98.671, 98.671, 127.453
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.243
Rwork	0.241
R-free	0.27300
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	XTALVIEW

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.060	0.240
Number of reflections	27558
<I/σ(I)>		3.6
Completeness [%]	95.5	98.4
Redundancy	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	289	PEG3350, ethyleneglycol, tris, DDMG, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 289K