1YRW

Crystal Structure of E.coli ArnA Transformylase Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Collection date	2004-03-09
Detector	RIGAKU RAXIS IV
Spacegroup name	C 2 2 21
Unit cell lengths	59.103, 113.253, 124.946
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.760 - 1.700
R-factor	0.1981
Rwork	0.198
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fmt
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.800	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.025	0.156
Number of reflections	46093
<I/σ(I)>	18.1	7.2
Completeness [%]	99.0	98.8
Redundancy	2.8	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.25	298	12% PEG 6000, 0.5 M NaCl, 100 mM HEPES pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.25	298	12% PEG 6000, 0.5 M NaCl, 100 mM HEPES pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K