1YRV
Novel Ubiquitin-Conjugating Enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-01-27 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 47.968, 74.009, 119.733 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.130 - 2.180 |
R-factor | 0.2441 |
Rwork | 0.244 |
R-free | 0.29400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2e2c |
RMSD bond length | 0.013 |
RMSD bond angle | 1.500 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.010 | 2.260 |
High resolution limit [Å] | 2.181 | 2.180 |
Rmerge | 0.042 | 0.336 |
Number of reflections | 11277 | |
<I/σ(I)> | 20.3 | 4.1 |
Completeness [%] | 98.2 | 98.1 |
Redundancy | 5.78 | 5.22 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 298 | 10% PEG MME 5000, 5% Tacsimate, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K, pH 7.00 |