1YRV
Novel Ubiquitin-Conjugating Enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-27 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 47.968, 74.009, 119.733 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.130 - 2.180 |
| R-factor | 0.2441 |
| Rwork | 0.244 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e2c |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.010 | 2.260 |
| High resolution limit [Å] | 2.181 | 2.180 |
| Rmerge | 0.042 | 0.336 |
| Number of reflections | 11277 | |
| <I/σ(I)> | 20.3 | 4.1 |
| Completeness [%] | 98.2 | 98.1 |
| Redundancy | 5.78 | 5.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 10% PEG MME 5000, 5% Tacsimate, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K, pH 7.00 |
