1YRM
Crystal Structure of an RNA duplex containing site specific 2'-amine substitutions at a C-A mismatch
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-03-15 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 43.000, 43.000, 122.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 7.980 - 2.500 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NDB ENTRY AR0002 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.900 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.500 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.044 | 0.255 |
Number of reflections | 5305 | |
<I/σ(I)> | 17.19 | 3.77 |
Completeness [%] | 95.2 | 75.2 |
Redundancy | 13.09 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 273 | MPD, cacodylate, spermidine, strontium chloride, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | spermidine | ||
3 | 1 | 1 | strontium chloride | ||
4 | 1 | 1 | cacodylate | ||
5 | 1 | 2 | strontium chloride | ||
6 | 1 | 2 | MPD | ||
7 | 1 | 2 | cacodylate |