1YRK

The C2 Domain of PKC is a new Phospho-Tyrosine Binding Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-01
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	37.272, 41.856, 88.463
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.350 - 1.700
R-factor	0.176
Rwork	0.176
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.700
Number of reflections	15254
Completeness [%]	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	PEG4K, PEG200, potassium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K