1YRI
Chicken villin subdomain HP-35, N68H, pH6.4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2004-10-24 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 31.980, 31.980, 57.027 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.000 |
| R-factor | 0.182 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yrf |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.020 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | EPMR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.040 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Number of reflections | 18869 | |
| <I/σ(I)> | 14.4 | 4 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 25.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.4 | 293 | 1ul 100mg/ml peptide plus 1ul (200mM AmOAc, 100mM NaCitrate, 30% PEG4000), pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 6.40 |
